This study involved 74 healthy Nigerian adults comprising 14 immunised (IM) and 60 nonimmunised (NIM) against HBV infection. They were sero-assayed for HBsAg, HBeAg, anti- HBe, anti-HBc, anti-HBs and anti-HCV using 3rd generation ELISA kits. HBsAg, HBeAg and anti-HBe, anti-HBc, anti-HbsAg and anti-HCV were seen in 39.2%, 13.5%, 2.7%, 12.2%, 35.1%, and 10.8%, respectively. Only the detection rate of HBeAg was greater than anti-HBe, p< 0.005 while those of HBsAg, HBeAg, anti-HBe and anti-HBc were higher among the NIM (p<0.05 each) compared to the IM. In addition, only detection rates of anti-HBe and anti-HBc were comparatively greater among male subjects (p<0.05). Serological diagnoses suggestive of early non-infective phase, infectivity (only among NIM), carrier state and no exposure to HBV infection were observed in 23%, 13.5%, 2.7% and 60.8% of all subjects, respectively. Further, 35.1% of the subjects had possible exposure to two HBV serotypes. Only 14.3% of the IM had evidence of early phase HBV infection compared to 45% of the NIM group in its different phases (p<0.05). Co-infection of both HBV and HCV was observed in 1.3% of all subjects but mainly among NIM. In conclusion, various types of serological patterns of HBV infection including dual HBV serotypes are present in Nigerian adults while a very high proportion of citizens remain unexposed to the infection. Immunisation against HBV protects against the acquisition and serological manifestation of infection.